Pitondo-Silva, AndréSantos, Adolfo Carlos Barreto [UNESP]Jolley, Keith A.Leite, Clarice Queico Fujimura [UNESP]Darini, Ana Lúcia da Costa2014-05-272014-05-272013-04-01Journal of Microbiological Methods, v. 93, n. 1, p. 42-48, 2013.0167-70121872-8359http://hdl.handle.net/11449/74936This study describes the comparison of three methods for genotyping of Mycobacterium tuberculosis, namely MIRU-VNTR (mycobacterial interspersed repetitive units-variable number of tandem repeats), spoligotyping and, for the first time, MLST (Multilocus Sequence Typing). In order to evaluate the discriminatory power of these methods, a total of 44 M. tuberculosis isolates obtained from sputum specimens of patients from Brazil were genotyped. Among the three methods, MLST showed the lowest discriminatory power compared to the other two techniques. MIRU-VNTR showed better discriminatory power when compared to spoligotyping, however, the combination of both methods provides the greatest level of discrimination and therefore this combination is the most useful genotyping tool to be applied to M. tuberculosis isolates. © 2013 Elsevier B.V.42-48engM. tuberculosisMIRU-VNTRMLSTSpoligotypingadultbacterial genomebacterial strainbacterium identificationbacterium isolateBrazilcladisticscontrolled studygene clustergenetic variabilityhumanintermethod comparisonmolecular modelmolecular typingmultilocus sequence typingMycobacterium tuberculosisnonhumannucleotide sequencepriority journalsequence analysisvariable number of tandem repeatAdultAgedAged, 80 and overFemaleGenetic VariationHumansMaleMiddle AgedMolecular EpidemiologyMolecular TypingSensitivity and SpecificitySputumTuberculosis, PulmonaryYoung AdultArtigo10.1016/j.mimet.2013.01.020WOS:000317326800008Acesso aberto2-s2.0-848747326702-s2.0-84874732670.pdf2114570774349859