Silva, Bárbara V.M.Rodríguez, Blanca A.G.Sales, Goreti F.Sotomayor, Maria Del Pilar T. [UNESP]Dutra, Rosa F.2018-12-112018-12-112016-03-15Biosensors and Bioelectronics, v. 77, p. 978-985.1873-42350956-5663http://hdl.handle.net/11449/177571A nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix assembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and differential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and morphological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid carboxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical performance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1ngmL-1 (r=0.995, p«0.01), with a very low limit of detection (0.006ngmL-1). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with excellent affinity to cTnT binding (KD=7.3 10-13molL-1). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications.978-985engAcid carboxylic-3-pyrroleCardiac troponin TGrapheneMolecularly imprinted polymerPoint-of-care testingPyrroleArtigo10.1016/j.bios.2015.10.068Acesso aberto2-s2.0-849460177542-s2.0-84946017754.pdf