Nakaie, C. R.Silva, E. G.Cilli, Eduardo Maffud [UNESP]Marchetto, Reinaldo [UNESP]Schreier, S.Paiva, T. B.Paiva, ACM2014-05-202014-05-202002-01-01Peptides. New York: Elsevier B.V., v. 23, n. 1, p. 65-70, 2002.0196-9781http://hdl.handle.net/11449/34799Angiotensin II (AngII) and bradykinin (BK) derivatives containing the TOAC (2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid) spin label were synthesized by solid phase methodology. Ammonium hydroxide (pH 10, 50degreesC, 1 h) was the best means for reverting nitroxide protonation occurring during peptide cleavage. EPR spectra yielded rotational correlation times for internally labeled analogs that were nearly twice as large as those of N-terminally labeled analogs. Except for TOAC(1)-AngII and TOAC(0)-BK, which showed high intrinsic activities, other derivatives were inactive in smooth muscle preparations. These active paramagnetic analogs may be useful for conformational studies in solution and in the presence of model and biological membranes. (C) 2002 Elsevier B.V. All rights reserved.65-70engangiotensin analogsbradykinin analogspeptide synthesisspin labeled peptidesTOAC spin labelelectron paramagnetic resonanceArtigo10.1016/S0196-9781(01)00580-0WOS:000173678100009Acesso restrito942434676246041657111822516411030000-0002-4767-0904