Castoldi, Robson Chacon [UNESP]Ozaki, Guilherme Akio TamuraGarcia, Thiago AlvesGiometti, Ines CristinaKoike, Tatiana Emy [UNESP]Camargo, Regina Celi Trindade [UNESP]dos Santos Pereira, João Domingos Augusto [UNESP]Constantino, Carlos José Leopoldo [UNESP]Louzada, Mário Jefferson Quirino [UNESP]Camargo Filho, José Carlos Silva [UNESP]Belangero, William Dias2019-10-062019-10-062019-01-01Lasers in Medical Science.1435-604X0268-8921http://hdl.handle.net/11449/189348The aim of the present study was to verify the effects of muscular strength training and growth hormone (GH) supplementation on femoral bone tissue by Raman spectroscopy (Raman), dual-energy X-ray absorptiometry (DXA), and mechanical resistance (F-max) analysis. A total of 40 male Wistar animals, 60 days old, were used. The animals were distributed into four groups: control (C), control with GH (GHC), muscular strength training (T), and muscular strength training with GH (GHT). Blood samples were collected for the quantification of creatine kinase (CK-MB) and the femurs were removed for analysis by Raman, DXA, and F-max. A more pronounced increase in the bone mineral components was verified in the T group, for all the variables obtained by the Raman (calcium, phosphate, amide, and collagen). In addition, for animals submitted to GH supplementation, there was a reduction in the variable bone mineral density (BMD) obtained by the DXA (p < 0.05). Finally, the animals that received GH supplementation presented a higher F-max, but without statistical significance (p > 0.05). It was concluded that animals that received GH supplementation demonstrated a decrease in BMD. In addition, T alone was able to promote increased calcium, phosphate, amide, and collagen compounds in bone tissue.engAnabolicsBone tissueGrowth hormoneMuscular strengthPhysical trainingArtigo10.1007/s10103-019-02821-5Acesso aberto2-s2.0-85068162315