Aranha, Andreza M.F. [UNESP]Giro, Elisa M.A. [UNESP]Souza, Pedro P.C. [UNESP]Hebling, Josimeri [UNESP]de Souza Costa, Carlos A. [UNESP]2014-05-272014-05-272006-09-01Dental Materials, v. 22, n. 9, p. 864-869, 2006.0109-5641http://hdl.handle.net/11449/69053Objective: The aim of this in vitro study was to evaluate the cytotoxicity of resin-modified glass-ionomer lining cements submitted to different curing regimes and applied to an immortalized odontoblast-cell line (MDPC-23). Methods: Forty round-shaped specimens of each experimental material (Fuji Lining LC and Vitrebond) were prepared. They were light-cured for the manufacturers' recommended time (MRT = 30 s), under-cured (0.5 MRT = 15 s), over-cured (1.5 MRT = 45 s) or allowed to dark cure (0 MRT). Sterilized filter papers soaked with either 5 μL of PBS or HEMA were used as negative and positive control, respectively. After placing the specimens individually in wells of 24-well dishes, odontoblast-like cells MDPC-23 (30,000 cells/cm2) were plated in each well and incubated for 72 h in a humidified incubator at 37 °C with 5% CO2 and 95% air. The cytotoxicity was evaluated by the cell metabolism (MTT assay) and cell morphology (SEM). Results: Fuji Lining LC was less cytotoxic than Vitrebond (p < 0.05) in all the experimental conditions. However, the cytotoxicity of Fuji Lining LC was noticeably increased in the absence of light-curing while the same was not observed for Vitrebond. The length of light-curing (15, 30 or 45 s) did not influence the toxicity of both lining materials when they were applied on the odontoblast-cell line MDPC-23. Significance: The light-activation plays an important role in reducing the cytotoxicity of Fuji Lining LC. Following the manufacturer' recommendation regarding the light-curing regime may prevent toxic effect to the pulp cells. © 2005 Academy of Dental Materials.864-869engCuring regimeCytotoxicityGlass-ionomer cementsHEMAOdontoblastCell cultureChemical bondsCuringGlassIonomersMorphologyDental cement2 hydroxyethyl methacrylate4 anisyltetrazolium blue4-anisyltetrazolium bluecoloring agentdentin bonding agentFuji glass ionomer lining cementFuji glass-ionomer lining cementglass ionomermethacrylic acid derivativeresintetrazoliumVitrabondanimalcell linecell shapechemistrydental surgerydrug effectlightmetabolismmouseodontoblastphase transitionradiation exposurescanning electron microscopyAnimalsCell Line, TransformedCell ShapeColoring AgentsDental Cavity LiningDentin-Bonding AgentsGlass Ionomer CementsLightMethacrylatesMiceMicroscopy, Electron, ScanningOdontoblastsPhase TransitionResins, SyntheticTetrazolium SaltsArtigo10.1016/j.dental.2005.11.015Acesso restrito2-s2.0-337466355674517484241515548