Sassaki, C. Y. [UNESP]Colodel, M. M. [UNESP]Ferreira, I. [UNESP]Nogueira, F. S.Lucheis, Simone Baldini [UNESP]Langoni, Hélio [UNESP]Rocha, Noeme Sousa [UNESP]2014-05-272014-05-272011-09-19Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 17, n. 3, p. 348-352, 2011.1678-91801678-9199http://hdl.handle.net/11449/132313Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofuorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more efective than PCR of lymph node aspirate culture. There was no statistical diference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node aspirate culture and PCR of hemoculture. All infected and uninfected animals had positive and negative results in at least one test. In conclusion, the association of several tests improves the efcacy of canine visceral leishmaniasis diagnosis.348-352engDiagnosisDogsVisceral leishmaniasisAnimal experimentAnimal parasitosisAspiration cytologyBlood cultureControlled studyDiagnostic testDifferential diagnosisDogImmunofluorescenceLaboratory diagnosisLeishmaniaLymph node biopsyNonhumanPolymerase chain reactionVisceral leishmaniasisAnimaliaCanis familiarisArtigo10.1590/S1678-91992011000300015S1678-91992011000300015WOS:000294438900014Acesso aberto2-s2.0-80052735361S1678-91992011000300015-en.pdf532607211851806760777359184692840000-0002-8188-8149