Cecchetto, Juliana [UNESP]Carvalho, Fernanda C. [UNESP]Santos, Adriano [UNESP]Fernandes, Flavio C. B. [UNESP]Bueno, Paulo R. [UNESP]2015-10-212015-10-212015-07-05Sensors And Actuators B-chemical. Lausanne: Elsevier Science Sa, v. 213, p. 150-154, 2015.0925-4005http://hdl.handle.net/11449/129089NS1, a non-structural dengue protein, has been used as a biomarker for the detection of viremia in dengue diagnosis. In this work, an impedimetric label-free immunosensor based on an anti-NS1 modified gold electrode was developed. To immobilize anti-NS1, a mixed self-assembled monolayer consisting of 11-mercaptoundecanoic acid (for covalent anti-NS1 attachment) and 6-mercaptohexanol (as a spacer) was prepared. The impedance spectra were recorded in the presence of a redox probe ( [Fe(CN)(6)](3-/4-)) to monitor changes in charge-transfer resistance associated with target binding both in PBS and neat serum. A calibration curve was constructed in order to obtain analytical parameters. NS1 was diluted in PBS to final concentrations in the linear range of 0.01-2.00 mu g mL(-1) with a sensitivity of 14.1 percentage decade(-1) (R similar to 0.99) and a limit of detection (LOD) of 3 ng mL(-1). A linear range 0.01-1.00 mu g mL(-1) of NS1 diluted in neat serum was also obtained (R similar to 0.98) with a sensitivity of 10.4 percentage decade(-1) and a LOD of 30 ng mL(-1). The results open new insights into the potential use of this biosensor for point-of-care and bedside applications for dengue diagnosis.150-154engDengueNS1 proteinElectrochemical impedance spectroscopy (EIS)Label-free biosensorBlood serumArtigo10.1016/j.snb.2015.02.068WOS:000352152500020Acesso restrito