Ferrarese, MLLRodrigues, J. D.Ferrarese, O.2014-05-202014-05-202000-03-01Plant Biology. Stuttgart: Georg Thieme Verlag, v. 2, n. 2, p. 152-153, 2000.1435-8603http://hdl.handle.net/11449/17212The high performance liquid chromatography (HPLC) technique was applied to measure phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity in soybean (Glycine max L. Merril cv. BR16) roots. t-Cinnamate, the catalytic product of the PAL reaction was quantified at 275 nm by isocratic elution with methanol:water through an ODS(M) column. Comparative experiments were carried out with 1.0 mM ferulic acid, an inducer of PAL activity. The results suggest that liquid chromatography is a rapid and sensitive method to analyze PAL activity in non-purified extract.152-153engt-cinnamateHPLCPALsoybeanArtigo10.1055/s-2000-9162WOS:000086431400004Acesso restrito4211432128816409