Silva, Tereza Cristina Cardoso da [UNESP]Garcia, Juliana Albarracin [UNESP]2016-05-172016-05-172015-08-06GARCIA, Juliana Albarracin. Propriedades oncolíticas do vírus da cinomose canina: expressão da proteína 8 (TNFAIP8) em células de carcinoma mamário: Juliana Albarracin Garcia. -. 2015. 50 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária, 2015.http://hdl.handle.net/11449/138431Oncolytic virotherapy is a novel strategy for treatment of cancer in humans and companion animals as well. Canine distemper virus (CDV), a paramyxovirus, has proven to be oncolytic through induction of apoptosis in canine-derived tumour cells, yet the mechanism behind this inhibitory action is poorly understood. In this study, three human mammary tumour cell lines and one canine-derived adenofibrossarcoma cell line were tested regarding to their susceptibility to CDV infection, apoptosis, mitochondrial membrane potential (MMP) and expression of tumour necrosis factor-alpha-induced protein 8 (TNFAIP8). CDV replication induced cytopathic effect and > 45% of infected cells were considered death and/or under late apoptosis/necrosis. TNFAIP8 and CDV M gene expression were positively correlated in all cell lines. In addition, MMP was highly associated with increase in virus titres (p<0.005). Thus, these results strongly suggest that both human and canine mammary tumour cells are potential candidates for studies concerning CDV-induced cancer therapy50 f.porCinomoseCãesMamas - CancerOncologia veterinariaFenotipoMarcadores biológicos de tumorFator de necrose de tumoralpha-induced protein 8Dissertação de mestradoAcesso aberto000864249http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/15-04-2016/000864249.pdf33004021075P8