Marconi, Camila [UNESP]Andrade Ramos, Bruna Ribeiro de [UNESP]Peraçoli, José Carlos [UNESP]Donders, Gilbert G. G.Silva, Marcia da [UNESP]2014-05-202014-05-202011-06-01American Journal of Reproductive Immunology. Malden: Wiley-blackwell, v. 65, n. 6, p. 549-556, 2011.1046-7408http://hdl.handle.net/11449/12196ProblemWe compared the frequency of intra-amniotic infection in preterm labor (PL) with women not in labor, and correlated infection with amniotic fluid (AF) cytokines. Detailed identification of species, especially mycoplasmata, was tried to improve our understanding of the pathogenesis of PL.Method of studyAF from 20 women with PL and 20 controls were evaluated. Infection was detected by PCR for Mycoplasma hominis, Ureaplasma urealyticum and 16S rRNA bacterial gene, which was cloned and sequenced for bacterial identification. Interleukin (IL)-1 beta, IL-6, IL-8 and tumor necrosis factor (TNF)-alpha levels were measured by ELISA.ResultsFrequency of intra-amniotic infection is higher in PL (40.0%). Sequencing-based method identified Bacteroides fragilis, Prevotella bivia and Leptotrichia amnionii, in addition to Mycoplasma species detected by PCR. AF infection correlated with increased IL-1 beta and IL-6 levels.ConclusionThe frequency of intra-amniotic infection, especially M. hominis, in PL women who delivered with 7 days, is high and correlates with high IL-1 beta and IL-6 levels, but not IL-8.549-556engCytokinesintra-amniotic infectionMycoplasma hominisUreaplasma urealyticumArtigo10.1111/j.1600-0897.2010.00940.xWOS:000289896500003Acesso restrito8499437381595614