Grassi, Tony F.Camargo, Elaine A.Salvadori, Daisy Maria Favero [UNESP]Marques, Mariângela Esther Alencar [UNESP]Ribeiro, Daniel A.2014-05-202014-05-202007-03-01International Journal of Hygiene and Environmental Health. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 210, n. 2, p. 163-167, 2007.1438-4639http://hdl.handle.net/11449/12829Since chlorhexidine is effective against microorganisms, it is widely recommended in dentistry. However, studies have provided evidence that chlorhexidine is toxic for a variety of cell types. In order to identify potential genotoxins in different cell types, the purpose of this study was to investigate whether chlorhexidine digluconate is able to cause, in terms of DNA damage, alterations in leukocytes, liver, kidney and urinary bladder by the single cell gel (comet) assay. Ten male Wistar rats were divided into two groups: a negative control and the experimental group treated with 3 ml of 0.12% chlorhexidine digluconate by gavage once a day for 8 days. Statistically significant increases of DNA damage was observed in leukocytes and kidney cells of the chlorhexidine digluconate treated group as depicted by the mean tail moment. Taken together, the data indicate that leukocytes and kidney cells are potential targets for primary DNA damage following oral exposure to chlorhexidine digluconate as detected by single cell gel (comet) assay. (c) 2006 Elsevier GmbH. All rights reserved.163-167engchlorhexidineDNA damagekidneyliversingle cell gel (comet) assayurinary bladderArtigo10.1016/j.ijheh.2006.09.001WOS:000244785400009Acesso restrito50511187529809037528116925519142