VEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptors

Vilardi, Bruna Maria RodriguesBravo-Calderón, Diego MauricioBernabé, Daniel Galera [UNESP]Oliveira, Sandra Helena Penha de [UNESP]Oliveira, Denise Tostes2014-05-272014-05-272013-02-01Tumor Biology, v. 34, n. 1, p. 139-143, 2013.1010-42831423-0380http://hdl.handle.net/11449/74458The aim of this study was to investigate the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous cell carcinoma (OSCC) cell lines through norepinephrine-induced activation of beta-adrenergic receptors. Human OSCC cell lines (SCC-9 and SCC-25) expressing beta-adrenergic receptors were stimulated with different concentrations of norepinephrine (0.1, 1, and 10 μM) and 1 μM of propranolol, and analyzed after 1, 6, and 24 h. VEGF-C gene expression and VEGF-C production in the cell supernatant were evaluated by real-time PCR and by ELISA, respectively. The results showed that beta-adrenergic receptor stimulation by different concentrations of norepinephrine or blocking by propranolol did not markedly alter VEGF-C expression by SCC-9 and SCC-25 cells. VEGF-C protein levels produced by oral malignant cell lines after stimulation with different norepinephrine concentrations or blocking with propranolol was statistically similar (p > 0.05) to those of the control group (nonstimulated OSCC cell lines). Our findings suggest that stimulation of beta-adrenergic receptors by means of norepinephrine does not seem to modulate the VEGF-C expression in OSCC cell lines. These findings reinforce the need for further studies in order to understand the responsiveness of oral cancer to beta-adrenergic receptor stimulation or blockage, especially with regard to VEGF-C production. © 2012 International Society of Oncology and BioMarkers (ISOBM).139-143engAdrenergic receptorsOral cancerVEGFbeta adrenergic receptornoradrenalinpropranololvasculotropin Ccancer cellcancer cell culturecell line SCC 25cell line SCC 9controlled studyenzyme activationenzyme activityenzyme synthesishumanhuman cellin vitro studymouth squamous cell carcinomapriority journalprotein analysisprotein expressionprotein functionAdrenergic beta-AntagonistsCarcinoma, Squamous CellCell Line, TumorHumansMouth NeoplasmsNeurotransmitter AgentsNorepinephrinePropranololReceptors, Adrenergic, betaVascular Endothelial Growth Factor CVEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptorsArtigo10.1007/s13277-012-0521-1WOS:000313875400018Acesso restrito2-s2.0-84872783923