Gomes, Virgínia Juliani [UNESP]Rezeck Nunes, Priscila [UNESP]Haworth, Sarah McCannSandrim, Valéria Cristina [UNESP]Peraçoli, José Carlos [UNESP]Peraçoli, Maria Terezinha S. [UNESP]Carlström, Mattias2021-06-252021-06-252021-01-01Hypertension in Pregnancy.1525-60651064-1955http://hdl.handle.net/11449/207285Objective: To investigate whether the supernatant from monocytes of preeclamptic and normotensive pregnant women, cultured in vitro with silibinin, can modulate oxidative stress in HUVEC. Methods: Concentrations of IL-1β, IL-10, and TNF-α in monocyte culture supernatants were determined by ELISA. HUVEC and their supernatant cultures were employed for determination of NO, nitrite and nitrate, lipid peroxidation, and hemeoxygenase-1 (HO-1). Results: HUVEC treatment with supernatant of preeclamptic monocytes cultured with silibinin produced increased levels of nitrite, reduced lipid peroxidation, and increased HO-1. Conclusion: Supernatant of monocytes from preeclamptic women induce oxidative stress in HUVEC which can be reduced by silibinin treatment. Abbreviations: DAF-FMTM, Diaminofluorescein-FM; EDTA, Ethylenediaminetetraacetic acid; HO-1, heme oxygenase-1; HPLC, high-performance liquid chromatography; HUVEC, human umbilical vein endothelial cell; MDA, malondialdehyde; NO, nitric oxide; NT, normotensive; PE, preeclampsia; ROS, reactive oxygen species; Sb, silibinin.engHUVECmonocytesoxidative stresspreeclampsiasilibininArtigo10.1080/10641955.2021.18842582-s2.0-85100838923