Pérez-Pacheco, Cindy Grace [UNESP]Fernandes, Natalie Ap. Rodrigues [UNESP]Primo, Fernando Lucas [UNESP]Tedesco, Antonio ClaudioBellile, EmilyRetamal-Valdes, BelenFeres, MagdaGuimarães-Stabili, Morgana Rodrigues [UNESP]Rossa, Carlos [UNESP]2021-06-252021-06-252021-05-01Clinical Oral Investigations, v. 25, n. 5, p. 3217-3227, 2021.1436-37711432-6981http://hdl.handle.net/11449/206763Objective: Assess a single local application of curcumin-loaded nanoparticles as an adjunct to scaling and root planing (SRP) in nonsurgical periodontal treatment (NPT). Materials and methods: Twenty healthy subjects with periodontitis received SRP+PLGA/PLA nanoparticles loaded with 50 μg of curcumin (N-Curc) or SRP+empty nanoparticles. Probing pocket depth (PPD), clinical attachment level (CAL), and bleeding on probing (BOP) were monitored at baseline, 30, 90, and 180 days. IL-1α, IL-6, TNFα, and IL-10 in the gingival crevicular fluid (GCF) were assessed by ELISA, and counts of 40 bacterial species were determined by DNA hybridization at baseline, 3, 7, and 15 days post-therapy. Results: PPD, CAL, and BOP were similarly and significantly improved in both experimental groups. There was no difference in GCF cytokine levels between experimental groups, although IL-6 was decreased at 3 days only in the N-Curc group. NPT reduced counts of red complex bacterial species in both groups. Veillonella Parvula counts increased significantly only in N-Curc group at 7 days, whereas Aggregatibacter actinomycetemcomitans counts increased significantly only in the control group from day 3 to day 15. Conclusion: We conclude that a single local administration of nanoencapsulated curcumin in periodontally diseased sites had no additive benefits to NPT. Clinical relevance: Our results showed that a single local application of curcumin-loaded nanoparticles associated with nonsurgical periodontal therapy did not improve clinical outcomes. Hence, our findings do not support the use of curcumin as an adjunct to nonsurgical periodontal therapy.3217-3227engCurcuminDental ScalingNanoparticlesPeriodontitisArtigo10.1007/s00784-020-03652-32-s2.0-85094683853