Machado, R. Z.Valadao, CAAMelo, W. R.Alessi, A. C.2014-05-202014-05-201994-11-01Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 27, n. 11, p. 2591-2598, 1994.0100-879Xhttp://hdl.handle.net/11449/377521. We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis.2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay.2591-2598engBABESIA BOVISBABESIA BIGEMINAMEROZOITE ISOLATIONISOLATION OF BABESIA-BIGEMINA AND BABESIA-BOVIS MEROZOITES BY AMMONIUM-CHLORIDE LYSIS OF INFECTED ERYTHROCYTESArtigoWOS:A1994PQ37600012Acesso restrito3254990612451836