Pereira, Wendell JacintoMelo, , Arthur Tavares De OliveiraCoelho, Alexandre Siqueira GuedesRodrigues, Fabiana AparecidaMamidi, SujanAlencar, Sérgio Amorim DeLanna, Anna CristinaValdisser, Paula Arielle Mendes RibeiroBrondani, ClaudioNascimento-júnior, Ivanildo Ramalho DoBorba, Tereza Cristina De OliveiraVianello, Rosana Pereira2019-10-032019-10-032019Genetics and Molecular Biology. Sociedade Brasileira de Genética, n. ahead, p. -, 2019.1415-4757http://hdl.handle.net/11449/183765Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.-engPhaseolus vulgariswater deficitfunctional genomicsSingle Nucleotide Polymorphism (SNP)Artigo10.1590/1678-4685-gmb-2018-0259S1415-47572019005032102Acesso abertoS1415-47572019005032102.pdf