Synthesis of the peptide Ac-Wahx-KTTKS and evaluation of the ability to induce in vitro collagen synthesis

Danila, A. S. Guglielmi [UNESP]Ariane, M. Martinelli [UNESP]Nathália, C. Rissi [UNESP]Eduardo, M. Cilli [UNESP]Christiane, P. Soares [UNESP]Leila, A. Chiavacci [UNESP]2018-12-112018-12-112016-06-01Protein and Peptide Letters, v. 23, n. 6, p. 544-547, 2016.1875-53050929-8665http://hdl.handle.net/11449/168728In this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac-Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%. In the cytotoxicity assay, we observed non-toxic effects for Ac-WAhx-KTTKS at concentrations below 600 ôM for HaCaT and 500 ôM for HepG2 cells, respectively. After 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. In conclusion, the Ac-Wahx-KTTKS peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging.544-547engAc-WAhx-KTTKSAntiagingCollagen biosynthesisCytotoxicityPeptide synthesisSkin functions.Synthesis of the peptide Ac-Wahx-KTTKS and evaluation of the ability to induce in vitro collagen synthesisArtigoAcesso restrito2-s2.0-84974735668