Toscano, Ilda A. S. [UNESP]Ribeiro, M. Lúcia [UNESP]Rocha, Julio C. [UNESP]Nunes, Gilvanda S.Barceló, Damiá2014-05-202014-05-202000-08-01Química Nova. Sociedade Brasileira de Química, v. 23, n. 4, p. 466-471, 2000.0100-4042http://hdl.handle.net/11449/27569ELISAs have been applied to pesticide residue analysis due to their high sensitivity and selectivity. However, some ELISAs performance may be affected by matrix components. In this work, ELISA for carbaryl in water samples was checked for interference by naturally occurring fulvic acids. The results suggested that the high fulvic acid concentration ( ³30 mg L-1) and acidic pH conditions (pH 4.0) interfere with the signal detection decreasing the method sensitivity. A dilution of the samples and adjust to pH 8.0 are appropriate to minimize the matrix interferences in the ELISA method. Good correlation between ELISA and HPLC-DAD results was observed.466-471porELISAcarbarylfulvic acidsArtigo10.1590/S0100-40422000000400007S0100-40422000000400007Acesso abertoS0100-40422000000400007.pdf