Sannomiya, M.Rodrigues, C. M.Coelho, R. G.Santos, Lourdes Campaner dos [UNESP]Hiruma-Lima, C. A.Brito, ARSVilegas, Wagner [UNESP]2014-05-202014-05-202004-04-30Journal of Chromatography A. Amsterdam: Elsevier B.V., v. 1035, n. 1, p. 47-51, 2004.0021-9673http://hdl.handle.net/11449/17758The methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3-O-alpha-L-arabinoside, quercetin-3-O-beta-D-galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3-O-alpha-L-arabinoside (95 mg), quercetin-3-O-beta-D-galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by H-1 NMR, C-13 NMR and UV analyses. (C) 2004 Published by Elsevier B.V.47-51engcounter-current chromatographyByrsonima crassaplant materialsflavonoidsamentoflavoneArtigo10.1016/j.chroma.2004.02.050WOS:000220790800007Acesso restrito353825364060297738145049013868440000-0002-8645-37770000-0003-3032-2556