de Matos, Renata Prandini Adum [UNESP]Sichero, LauraMansur, Isabela Mazuco [UNESP]do Bonfim, Caroline Measso [UNESP]Bittar, Cíntia [UNESP]Nogueira, Rodrigo LacerdaKüpper, Daniel SalgadoValera, Fabiana Cardoso PereiraNogueira, Maurício LacerdaVilla, Luisa LinaCalmon, Marilia Freitas [UNESP]Rahal, Paula [UNESP]2014-05-272014-05-272013-06-01Infection, Genetics and Evolution, v. 16, p. 282-289.1567-13481567-7257http://hdl.handle.net/11449/75522There are few studies about the distribution of natural molecular variants of low-risk HPVs. Our aim was to evaluate the E6 early gene variability among HPV-6 and HPV-11 isolates detected in recurrent respiratory papillomatosis (RRP) samples obtained in a cohort of Brazilian patients. We also performed a phylogenetic analysis in order to compare nucleotide sequences identified in our study with previously reported isolates from different anatomic sites (laryngeal papillomas, genital warts, cervical cancer and anal swabs) obtained from other parts of the world to determine the phylogenetic relationships of variants detected in Brazil. The complete coding region of the E6 gene of 25 samples was cloned and sequenced: 18 isolates of HPV-6 (72%) and 7 isolates of HPV-11 (28%). A total of four different HPV-6 genomic variants and two HPV-11 genomic variants was identified. It was not possible to correlate specific variants with disease severity. Phylogenetic trees for both HPV types were constructed enclosing both E6 sequences detected in our study and formerly published sequences. In both phylogenetic trees, the sequences from Brazil did not group together. We could not establish a geographical association between HPV-6 or HPV-11 variants, unlike HPV-16 and HPV-18. © 2013 Elsevier B.V.282-289engHPV variantsHPV-11HPV-6Phylogenetic analysisRecurrent respiratory papillomatosisprotein E6adolescentadultamino acid substitutionBrazilchildclinical articlecohort analysiscondyloma acuminatumcontrolled studydisease severityE6 early genefemalegenetic variabilityhumanHuman papillomavirus type 11Human papillomavirus type 6larynx papillomatosislung tumormalemolecular cloningnonhumannucleic acid analysisnucleotide sequencepapillomatosisphylogenetic treephylogenypoint mutationpolymerase chain reactionpreschool childpriority journalschool childsequence analysistumor recurrenceuterine cervix cancervirus isolationArtigo10.1016/j.meegid.2012.12.033WOS:000320569500037Acesso aberto2-s2.0-848778177882-s2.0-84877817788.pdf79910823626712120000-0001-5693-6148