Coradi, Gilberto Victor [UNESP]Da Visitação, Viviane Loiola [UNESP]De Lima, Evandro Antônio [UNESP]Saito, Larissa Yumi Tsuchida [UNESP]Palmieri, Darío Abel [UNESP]Takita, Marco AurélioOliva Neto, Pedro de [UNESP]De Lima, Valéria Marta Gomes [UNESP]2014-05-272014-05-272013-06-01Annals of Microbiology, v. 63, n. 2, p. 533-540, 2013.1590-42611869-2044http://hdl.handle.net/11449/75496Lipase production by Trichoderma harzianum was evaluated in submerged fermentation (SF) and solid-state fermentation (SSF) using a variety of agro-industrial residues. Cultures in SF showed the highest activity (1.4 U/mL) in medium containing 0.5 % (w/v) yeast extract, 1 % (v/v) olive oil and 2.5 C:N ratio. This paper is the first to report lipase production by T. harzianum in SSF. A 1:2 mixture of castor oil cake and sugarcane bagasse supplemented with 1 % (v/w) olive oil showed the best results among the cultures in SSF (4 U/g ds). Lipolytic activity was stable in a slightly acidic to neutral pH, maintaining 50 % activity after 30 min at 50 C. Eighty percent of the activity remained after 1 h in 25 % (v/v) methanol, ethanol, isopropanol or acetone. Activity was observed with vegetable oils (olive, soybean, corn and sunflower) and long-chain triacylglycerols (triolein), confirming the presence of a true lipase. The results of this study are promising because they demonstrate an enzyme with interesting properties for application in catalysis produced by fermentation at low cost. © 2012 Springer-Verlag and the University of Milan.533-540engCassava cakeCastor beanCorn huskLipaseSolid-state and submerged fermentationSugarcane bagasseTrichoderma harzianumGlycine maxHelianthusHypocrea lixiiManihot esculentaRicinus communisZea maysArtigo10.1007/s13213-012-0500-1WOS:000319074600014Acesso restrito2-s2.0-8487802043446389522635027440000-0001-9378-9036