Machado, SRGregorio, E. A.2014-05-202014-05-202001-02-01Tissue & Cell. Edinburgh: Churchill Livingstone, v. 33, n. 1, p. 72-77, 2001.0040-8166http://hdl.handle.net/11449/17230This paper reports on a study of the zinc iodide-osmium tetroxide method (ZIO) applicability to formaldehyde-glutaraldehyde prefixed extrafloral nectary tissues of Citharexylum mirianthum Cham. (Verbenaceae). The ZIO solution impregnates the dictyosome stacks and adjacent vesicles, smooth endoplasmic reticulum, nuclear envelope, multivesicular bodies, and peroxisomes. The use of this method greatly facilitates the observation and recognition of organelles in each nectary region. it also allows the correlation between structure and function in nectariferous cells. (C) 2001 Harcourt Publishers Ltd.72-77engZIO methodultrastructureextrafloral nectaryCitharexylum mirianthumVerbenaceaeArtigo10.1054/tice.2000.0157WOS:000167535700009Acesso restrito2-s2.0-00350879832653496390637757