Guimaraes, Morgana R. [UNESP]de Aquino, Sabrina Garcia [UNESP]Coimbra, Leila S. [UNESP]Spolidório, Luis Carlos [UNESP]Kirkwood, Keith L.Rossa, Carlos [UNESP]2013-09-302014-05-202013-09-302014-05-202012-02-01Innate Immunity. London: Sage Publications Ltd, v. 18, n. 1, p. 155-163, 2012.1753-4259http://hdl.handle.net/11449/15902Curcumin is a plant-derived dietary spice ascribed various biological activities. Curcumin therapeutic applications have been studied in a variety of conditions, but not on periodontal disease. Periodontal disease is a chronic inflammatory condition initiated by an immune response to micro-organisms of the dental biofilm. Experimental periodontal disease was induced in rats by injecting LPS in the gingival tissues on the palatal aspect of upper first molars (30 mu g LPS, 3 times/week for 2 weeks). Curcumin was administered to rats daily via oral gavage at 30 and 100 mg/kg body weight. Reverse transcriptase-qPCR and ELISA were used to determine the expression of IL-6, TNF-alpha and prostaglandin E-2 synthase on the gingival tissues. The inflammatory status was evaluated by stereometric and descriptive analysis on hematoxylin/eosin-stained sections, whereas modulation of p38 MAPK and NK-kappa B signaling was assessed by Western blot. Curcumin effectively inhibited cytokine gene expression at mRNA and protein levels, but NF-kappa B was inhibited only with the lower dose of curcumin, whereas p38 MAPK activation was not affected. Curcumin produced a significant reduction on the inflammatory infiltrate and increased collagen content and fibroblastic cell numbers. Curcumin potently inhibits innate immune responses associated with periodontal disease, suggesting a therapeutic potential in this chronic inflammatory condition.155-163engCurcumininflammationinnate immunityperiodontal diseaseArtigo10.1177/1753425910392935WOS:000299162600014Acesso restrito2640929291808415