Leme, Daniela Morais [UNESP]Grummt, TamaraHeinze, RitaSehr, AndreaSkerswetat, MatthiasRodrigues de Marchi, Mary Rosa [UNESP]Machado, Marcos Canto [UNESP]de Oliveira, Danielle PalmaMann-Morales, Maria Aparecida [UNESP]2014-05-202014-05-202011-11-01Ecotoxicology and Environmental Safety. San Diego: Academic Press Inc. Elsevier B.V., v. 74, n. 8, p. 2148-2155, 2011.0147-6513http://hdl.handle.net/11449/19878The designation of biodiesel as a green fuel has increased its commercialization and use, making its fate in the environment a matter of concern. Fuel spills constitute a major source of aquatic pollution and, like diesel spills, biodiesel can produce adverse effects on aquatic environments, animals and humans. The present study assessed cytotoxic effects of water systems contaminated with neat biodiesel and its diesel blends by means of different procedures on human T cell leukemia (Jurkat) and human hepatocellular carcinoma (HepG2) cells [detection of changes in mitochondrial membrane potential (Delta Psi(m)) using tetramethylrhodamine ethyl ester (TMRE), apoptosis recognition by Annexin V and impedance real-time cell analyzer (xCELLigence (TM) system)]. The data obtained showed concordance across the different bioassays, with cytotoxic effects observed as a dose-dependent response only for waters contaminated with pure diesel (D100) and B5 blend, which is characterized by a mixture of 95% diesel and 5% biodiesel. The data can also lead us to hypothesize that diesel accounts for the harmful effects observed, and that biodiesel does not worsen the impacts caused by diesel pollution. (C) 2011 Elsevier B.V. All rights reserved.2148-2155engPolycyclic aromatic hydrocarbonsCell proliferation and viabilityCell death processApoptosisNecrosisArtigo10.1016/j.ecoenv.2011.08.012WOS:000296939700003Acesso restrito