Giannoni, M. L.Foresti, F.Falcone, C.Tosta, P. A.2014-05-202014-05-201993-06-01Revista Brasileira de Genetica. Ribeirao Pret: Soc Brasil Genetica, v. 16, n. 2, p. 477-483, 1993.0100-8455http://hdl.handle.net/11449/35455In order to develop an efficient and low-cost technique for obtaining bird chromosome preparations, and to adapt the cytogenetic process of bird sexing for general use at zoos and breeding farms with the technical support of cytogenetics laboratories, we tested variants of the technique described by Giannoni et al. (Genet. Sel. Evol. 23: 123-125, 1991), based on the utilization of cellular material from growing feather pulp cultured in complete medium for six hours. Hanks' saline solution gave satisfactory performance as a substitute for complete medium, with no need to use PHA, serum of collagenase, when utilized in material obtained from feather pulp of Amazona amazonica (Psittacidae).477-483engArtigoWOS:A1993LJ96500020Acesso abertoWOSA1993LJ96500020.pdf