Uliana, Carolina V. [UNESP]Riccardi, Carla S. [UNESP]Tognolli, Joao O. [UNESP]Yamanaka, Hideko [UNESP]2014-05-202014-05-202008-01-01Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 19, n. 4, p. 782-787, 2008.0103-5053http://hdl.handle.net/11449/25839Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL(-1) and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1: 1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.782-787engbiosensorhepatitis C virusfractional factorial designArtigo10.1590/S0103-50532008000400024S0103-50532008000400024WOS:000256957100023Acesso abertoS0103-50532008000400024.pdf1923726000036625