Vilas-Bôas, Gislayne Trindade [UNESP]Lemos, Manoel Victor Franco [UNESP]2014-05-272014-05-272004-08-01Canadian Journal of Microbiology, v. 50, n. 8, p. 605-613, 2004.0008-4166http://hdl.handle.net/11449/67819Two hundred and eighteen Bacillus thuringiensis isolates from Brazil were characterized by the presence of crystal protein genes by PCR with primers specific to different cry and cyt genes. Among these isolates, 95 were selected according to their geographic origin for genetic characterization with the 16S rRNA gene, RAPD, and plasmid profile. Isolates containing cryl genes were the most abundant (48%) followed by the cry11 and cyt (7%) and cry8 genes (2%). Finally, 40.3% of the isolates did not produce any PCR product. The plasmid profile and RAPD analysis showed a remarkable diversity among the isolates of B. thuringiensis not observed in the 16S rRNA gene. These results suggest that the genetic diversity of B. thuringiensis species results from the influence of different ecological factors and spatial separation between strains generated by the conquest of different habitats.605-613eng16S rRNA geneBacillus thuringiensisCry genesEcologyGenetic diversityPlasmid profileRAPDBiodiversityCrystal structureGenesProteinsRNAGenetic characterizationSpatial separationBacteriaribosome RNARNA 16Sbacteriumgenetic variationplasmidpolymerase chain reactionbacterial genebacterium isolateBrazilcry genecyt genegenetic variabilitygeographic distributionhabitat structurenonhumanpriority journalrandom amplified polymorphic DNARNA geneBacterial ProteinsBacterial ToxinsDNA, RibosomalEndotoxinsHemolysin ProteinsMolecular Sequence DataPlasmidsPolymerase Chain ReactionRandom Amplified Polymorphic DNA TechniqueRNA, Ribosomal, 16SSequence Analysis, DNAVariation (Genetics)South AmericaWestern HemisphereWorldBacteria (microorganisms)PosibacteriaArtigo10.1139/w04-052WOS:000224734600007Acesso restrito2-s2.0-86442731127179273060624761