Carvalho, A. de B.Silva, A. L. daSilva, A. M. de A.Netto, A. J.Medeiros, T. T. B. deAraujo Filho, J. M.Agostini, D. L. da S. [UNESP]Oliveira, D. L. deMazzetto, S. E.Kotzebue, L. R.Oliveira, J. R.Oliveira, R. L.Bezerra, L. R.2020-12-102020-12-102019-10-01Small Ruminant Research. Amsterdam: Elsevier, v. 179, p. 56-63, 2019.0921-4488http://hdl.handle.net/11449/196278The objective of this study was to evaluate the effects of including slow-release urea microencapsulated in beeswax in the diet of adult sheep on intake, digestibility, and nitrogen (N) balance. Two microencapsulated system formulations were developed with ratios (wt/wt) of 1:2 (U(ME)1:2) and 1:2 + sulfur (U(ME)1:2 + S) between the core (urea) and encapsulant (beeswax). The U(ME)1:2 formulation had greater yield values, microencapsulation efficiency and thermal stability than U(ME)1:2 + S. The most efficient formulation (U(ME)1:2) was added to the diet of twenty-four uncastrated male sheep, adult, crossbred Santa Ines, 9 months old with an average body weight of 28.2 kg +/- 0.6 kg. The experimental arrangement was a completely randomized design in which the animals were distributed into four treatments that included different levels of urea microencapsulated in beeswax, U(ME)1:2 without S (0, 1.5, 3.0 and 4.5% in total dry matter (DM)), and six replicates in a total experimental period of 19 days, with 14 days for adaptation. The inclusion of urea microencapsulated in beeswax did not affect (P > 0.05) DM, crude protein, ether extract, ash, total carbohydrate or crude energy intake or digestibility. In addition, the nonfibrous carbohydrate (NFC), neutral detergent fiber (NDF), and acid detergent fiber (ADF) intake, N intake, N excretion (urinary and fecal), N absorbed, N retained, N retention/N intake and N retained/N absorbed were not affected (P > 0.05) by the inclusion of urea micro-encapsulated in beeswax. However, there was a linear increase in NDF (P = 0.031) and ADF (P = 0.004) digestibility as the inclusion level of encapsulated urea increased from 0 to 4.5%. The NFC digestibility showed a quadratic increase (P < 0.001) with the greatest digestibility at 3.0% microencapsulated urea. Beeswax was shown to be an efficient microencapsulant to obtain microparticles containing urea, and the formulation of U(ME)1:2 without the addition of S is recommended since it showed better yield and efficiency than the formulation with S. The inclusion of up to 4.5% urea microencapsulated in the lipid matrix of beeswax is recommended to improve NDF and ADF digestibility without affecting intake and N balance.56-63engIntakeMicrospheresN-NH3Nitrogen balanceArtigo10.1016/j.smallrumres.2019.09.005WOS:000493213100010