Pedraza-Ordoñez, Francisco [UNESP]Yamatogi, Ricardo Seidi [UNESP]Araújo Júnior, João Pessoa [UNESP]Rocha, Noeme Souza [UNESP]Alessi, Antonio Carlos [UNESP]2016-07-072016-07-072013Journal of Veterinary Science & Technology, v. 4, n. 5, p. 1-5, 2013.2157-7579http://hdl.handle.net/11449/141083Background: Bovine herpes virus 5 (BoHV-5) is a neurovirulent alpha herpes virus that causes fatal meningoencephalitis and huge economic losses in the livestock and associated industries worldwide; its diagnosis in the laboratory is essential for disease control, immunologic surveillance and effective monitoring programs in endemic areas. Materials and Methods: In this study, brain samples from 21 Colombian cattle that died of neurological disease, were negative for rabies and were diagnosed with nonspecific encephalitis were processed by real-time PCR (qPCR) in the laboratory of virology at the Institute of Biosciences, Sao Paulo State University, Brazil, to detect BoHV-5 genetic material in tissue preserved in paraffin blocks. Tissues were considered positive for BoHV-5 if amplification occurred at or before 30 PCR cycles, inconclusive if amplification occurred between 30 and 40 cycles and negative otherwise. Results and Conclusion: Two cases were positive, demonstrating that qPCR may be useful for confirming herpes encephalitis in acid formalin-fixed tissues with an inconclusive diagnosis.1-5engBovine herpes virus 5Real-time PCRDiagnosisBoHV-5Artigo10.4172/2157-7579.1000145Acesso abertoISSN2157-7579-2013-04-05-01-05.pdf60777359184692840000-0002-8188-8149