Borecký, JiříNogueira, Fábio T. S.De Oliveira, Kívia A. P.Maia, Ivan de Godoy [UNESP]Vercesi, Aníbal E.Arruda, Paulo2014-05-272014-05-272006-03-01Journal of Experimental Botany, v. 57, n. 4, p. 849-864, 2006.0022-09571460-2431http://hdl.handle.net/11449/68788The simultaneous existence of alternative oxidases and uncoupling proteins in plants has raised the question as to why plants need two energy-dissipating systems with apparently similar physiological functions. A probably complete plant uncoupling protein gene family is described and the expression profiles of this family compared with the multigene family of alternative oxidases in Arabidopsis thaliana and sugarcane (Saccharum sp.) employed as dicot and monocot models, respectively. In total, six uncoupling protein genes, AtPUMP1-6, were recognized within the Arabidopsis genome and five (SsPUMP1-5) in a sugarcane EST database. The recombinant AtPUMP5 protein displayed similar biochemical properties as AtPUMP1. Sugarcane possessed four Arabidopsis AOx1-type orthologues (SsAOx1a-1d); no sugarcane orthologue corresponding to Arabidopsis AOx2-type genes was identified. Phylogenetic and expression analyses suggested that AtAOx1d does not belong to the AOx1-type family but forms a new (AOx3-type) family. Tissue-enriched expression profiling revealed that uncoupling protein genes were expressed more ubiquitously than the alternative oxidase genes. Distinct expression patterns among gene family members were observed between monocots and dicots and during chilling stress. These findings suggest that the members of each energy-dissipating system are subject to different cell or tissue/organ transcriptional regulation. As a result, plants may respond more flexibly to adverse biotic and abiotic conditions, in which oxidative stress is involved. © The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.849-864engAlternative oxidaseExpression profileOxidative stressUncoupling proteinsGenesPlants (botany)ProteinsSugar caneTissueDicotsMonocotsEnergy dissipationalternative oxidasecarrier proteinion channelmembrane proteinmessenger RNAmitochondrial proteinmitochondrial uncoupling proteinoxidoreductasevegetable proteinamino acid sequenceArabidopsisbiologycoldcomparative studyenzymologygene expression profilinggeneticsgenomemetabolismmitochondrionmolecular geneticsmultigene familyphylogenyphysiologyreverse transcription polymerase chain reactionsequence alignmentsequence analysissugarcaneAmino Acid SequenceCarrier ProteinsColdComputational BiologyGene Expression ProfilingGenome, PlantIon ChannelsMembrane ProteinsMitochondriaMitochondrial ProteinsMolecular Sequence DataMultigene FamilyOxidoreductasesPhylogenyPlant ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSaccharumSequence AlignmentSequence Analysis, ProteinEnergyGenotypesPlant TissuesPlantsSugar CaneArabidopsis thalianaDicotyledoneaeLiliopsidaSaccharum hybrid cultivarSaccharum sp.Artigo10.1093/jxb/erj070Acesso restrito2-s2.0-336448978878649222099176162