Ferreira, V. S.Zanoni, Maria Valnice Boldrin [UNESP]Fogg, A. G.2014-05-202014-05-201997-09-01Microchemical Journal. San Diego: Academic Press Inc. Jnl-comp Subscriptions, v. 57, n. 1, p. 115-122, 1997.0026-265Xhttp://hdl.handle.net/11449/31161A method was developed for the differential-pulse cathodic stripping voltammetric determination of ceftazidime with a hanging mercury drop electrode using its reduction peak at -0.43 V in Britton-Robinson buffer pH 4.0. The optimum accumulation potential and time were -0.15 V and up to 60 s, respectively. Linear calibration graphs were obtained from 1 x 10(-8) M and 1.5 x 10(-7) M. The limit of determination was calculated to be 5 x 10(-9) M. The coefficient of variation was 4% (n = 7) at 1 x 10(-7) M ceftazidime. The effect of various components of urine on the voltammetric response was studied, and creatinine, uric acid, urea, and glucose were shown to interfere in the method. Ceftazidime bound to human albumin gives a unique stripping peak at -0.48 V. Recoveries of 87% +/- 2% of the ceftazidime (n = 5) were obtained from urine spiked with 1.27 mu g ml(-1) using C-18 solid phase extraction cartridges. (C) 1997 Academic Press.115-122engArtigo10.1006/mchj.1997.1516WOS:A1997YG64600012Acesso restrito0000-0002-2296-1393