Souza, Lorena Brito de [UNESP]Aquino, Sabrina García de [UNESP]Souza, Pedro Paulo Chaves de [UNESP]Hebling, Josimeri [UNESP]Costa, Carlos Alberto de Souza [UNESP]2014-05-272014-05-272007-12-01American Journal of Dentistry, v. 20, n. 6, p. 400-404, 2007.0894-8275http://hdl.handle.net/11449/70206Purpose: To evaluate the cytotoxic effects of different concentrations of Chlorhexidine (Chx) to the odontoblast cell line MDPC-23. Methods: The odontoblast-like cells were seeded (30,000 cells/cm 2) in 60 wells of 24-well dishes and then incubated in contact with the following experimental and control solutions: Group 1: 0.0024% Chx; Group 2: 0.004% Chx; Group 3: 0.02% Chx; Group 4: Phosphate buffer saline solution (PBS, negative control); and Group 5: 0.06% H 2O 2 (positive control). Cell metabolic activity was measured by MTT assay and the cell morphology was analyzed by SEM. Results: The cytotoxic effects of Chx are dose-dependent. The reduction in the cell metabolism for Groups 1, 2, and 3 was 24.8%, 29.9% and 70.8%, respectively. No statistical difference was observed between the Groups 1 and 2 in which no significant cell morphology changes occurred. Consequently, it was concluded that 0.02% Chx solution presents high cytotoxicity to the odontoblast-like cells MDPC-23. On theother hand, 0.0024% and 0.004% Chx causes slight cytopathic effects to the cultured cells.400-404engbufferchlorhexidinecoloring agentdiagnostic agenthydrogen peroxideoxidizing agentsodium chloridetetrazoliumthiazole derivativethiazolyl bluetopical antiinfective agentunclassified druganimalcell adhesioncell linecell membranecell shapecomparative studydose responsedrug effectmetabolismmouseodontoblastpathologyultrastructureAnimalsAnti-Infective Agents, LocalBuffersCell AdhesionCell LineCell MembraneCell ShapeChlorhexidineColoring AgentsDose-Response Relationship, DrugHydrogen PeroxideMiceOdontoblastsOxidantsSodium ChlorideTetrazolium SaltsThiazolesArtigoWOS:000251825800011Acesso restrito2-s2.0-383491404584517484241515548