Pegorer, M. FigueiraEreno, R. L.Satrapa, R. A.Pinheiro, V. G.Trinca, L. A.Barros, C. M.2014-05-272014-05-272011-01-01Theriogenology, v. 75, n. 1, p. 17-23, 2011.0093-691Xhttp://hdl.handle.net/11449/72252The objective was to evaluate the effects of plasma progesterone (P4) concentrations and exogenous eCG on ovulation and pregnancy rates of pubertal Nellore heifers in fixed-time artificial insemination (FTAI) protocols. In Experiment 1 (Exp. 1), on Day 0 (7 d after ovulation), heifers (n = 15) were given 2 mg of estradiol benzoate (EB) im and randomly allocated to receive: an intravaginal progesterone-releasing device containing 0.558 g of P4 (group 0.5G, n = 4); an intravaginal device containing 1 g of P4 (group 1G, n = 4); 0.558 g of P4 and PGF2α (PGF; 150 μg d-cloprostenol, group 0.5G/PGF, n = 4); or 1 g of P4 and PGF (group 1G/PGF, n = 3). On Day 8, PGF was given to all heifers and intravaginal devices removed; 24 h later (Day 9), all heifers were given 1 mg EB im. In Exp. 2, pubertal Nellore heifers (n = 292) were treated as in Exp. 1, with FTAI on Day 10 (30 to 36 h after EB). In Exp. 3, pubertal heifers (n = 459) received the treatments described for groups 0.5G/PGF and 1G/PGF and were also given 300 IU of eCG im (groups 0.5G/PGF/eCG and 1G/PGF/eCG) at device removal (Day 8). In Exp. 1, plasma P4 concentrations were significantly higher in heifers that received 1.0 vs 0.588 g P4, and were significantly lower in heifers that received PGF on Day 0. In Exp. 2 and 3, there were no significant differences among groups in rates of ovulation (65-77%) or pregnancy (Exp. 2: 26-33%; Exp. 3: 39-43%). In Exp. 3, diameter of the dominant ovarian follicle on Day 9 was larger in heifers given 0.558 g vs 1.0 g P4 (10.3 ± 0.2 vs 9.3 ± 0.2 mm; P < 0.01). In conclusion, lesser amounts of P4 in the intravaginal device or PGF on Day 0 decreased plasma P4 from Days 1 to 8 and increased diameter of the dominant follicle on Day 9. However, neither of these nor 300 IU of eCG on Day 8 significantly increased rates of ovulation or pregnancy. © 2011.17-23engBos indicusFixed-time artificial inseminationNellore heifersProgesteronechorionic gonadotropindrug derivativeestradiolestradiol 3-benzoateestradiol benzoateprogesteroneprostaglandin Fanimalanimal diseaseartificial inseminationbloodcattleclinical trialcontrolled clinical trialcontrolled studydrug effectfemalemethodologyovulationovulation inductionphysiologypregnancypregnancy raterandomized controlled trialAnimalsCattleChorionic GonadotropinEstradiolFemaleInsemination, ArtificialOvulationOvulation InductionPregnancyPregnancy RateProstaglandins FArtigo10.1016/j.theriogenology.2010.07.005Acesso restrito2-s2.0-7864986079037204893664279557795883009987806