Tamayo, Abel I. BalbinRizo, Laura S. Lópezde Armas, Madelin BlancoFerreira, Antonio Aparecido Pupim [UNESP]Manzani, DaniloYamanaka, Hideko [UNESP]Guas, Ana Margarita Esteva2020-12-122020-12-122020-03-01Microchemical Journal, v. 153.0026-265Xhttp://hdl.handle.net/11449/201371This study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10− 12 molL−1 could be detected.engDNA biosensorsGenosensorHIV-1HybridizationImpedimetricProviral DNAArtigo10.1016/j.microc.2019.1044622-s2.0-85075892190