Mataqueiro, Maria Isabel [UNESP]Okada Nakaghi, Laura Satiko [UNESP]Yamada, Patricia Kii [UNESP]Ferraz, Guilherme de Camargo [UNESP]Queiroz-Neto, Antonio de [UNESP]DeOliveira, Georgino Honorato [UNESP]2014-12-032014-12-032014-02-01Journal Of Toxicology And Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 77, n. 1-3, p. 125-132, 2014.1528-7394http://hdl.handle.net/11449/111461The aim of this study was to determine the no-observable-adverse-effect concentration (NOAEC) for trichlorfon, an antiparasitic agent used in aquaculture, in Piractus mesopotamicus (pacu) using acetylcholinesterase (AChE) activity as an end point. Fish were exposed 24 h/d for 15 d to different concentrations of trichlorfon in tanks of water for which a curve of dissipation was previously determined. Analysis of trichlorfon in water and fish plasma using gas chromatography with electron capture detection (GC-ECD) enabled measurement of limit of detection (LOD) and limit of quantification (LOQ), respectively, to be 3 and 10 ppb. Thirty-six hours after trichlorfon dilution in water, the concentration was below the LOD, and data showed that plasma concentrations did not exceed the LOQ. Apart from the 6.25 g/L, all concentrations of trichlorfon significantly inhibited plasma and brain AChE activity compared to controls. The AChE activity levels returned to control values in 7 d. These data may be useful to determine the concentration of trichlorfon that destroys parasites without producing adverse effects in fish.125-132engArtigo10.1080/15287394.2013.866928WOS:000331523300012Acesso restrito9203413733944127