Ferreira-Silva, ValériaPrimo, Fernando L. [UNESP]Baqui, Munira M.A.Magalhães, Danielle A.R.Orellana, Maristela D.Castilho-Fernandes, AndrielleCruz, Mario C.Câmara, Niehls O. S.Covas, Dimas T.Tedesco, Antonio C.2018-12-112018-12-112018-08-01Stem Cell Reviews and Reports, v. 14, n. 4, p. 585-598, 2018.1558-68041550-8943http://hdl.handle.net/11449/177096The purpose of the present study was to evaluate the neural protein expression pattern of human multipotent mesenchymal stromal cells (hMSCs) treated with forskolin (free-form/FF). The study investigated forskolin’s capacity to enhance intracellular levels of cyclic adenosine monophosphate (cAMP) by activating adenylate cyclase and probably by inducing neuron-like cells in vitro. In addition, because nanotechnology is a growing field of tissue engineering, we also assessed the action of a new system called the nanostructured-forskolin (NF) to examine the improvement of drug delivery. Afterwards, the cells were submitted to low-level laser irradiation to evaluate possible photobiostimulatory effects. Investigations using the immunofluorescence by confocal microscopy and Western blot methods revealed the expression of the neuronal marker β-tubulin III. Fluorescence intensity quantification analysis using INCell Analyzer System for β-tubulin III was used to examine significant differences. The results showed that after low-level laser irradiation exposure, there was a tendency to increase the β-tubulin III expression in all groups, as expected in the photobiostimulation process. Notably, this process induced for irradiation was more pronounced in irradiated nanoforskolin cells (INF) compared to non-irradiated free-forskolin control cells (NFFC). However, there was also an increase in β-tubulin III protein expression in the groups: irradiated nanocontrol cells (INC) compared to non-irradiated free-forskolin control cells (NFF) and after treatment with non-irradiated free-forskolin (NFF) and non-irradiated nanoforskolin (NNFC). We concluded that the methods using low-level laser irradiation and/or nanoparticles showed an up-regulation of neural-protein expression in hMSCs that could be used to facilitate cellular therapy protocols in the near future.585-598engForskolinLow-intensity laser irradiationMultipotent mesenchymal stromal cellsNano-drug deliveryNanotechnologyβ-Tubulin IIIArtigo10.1007/s12015-017-9796-3Acesso aberto2-s2.0-850419178882-s2.0-85041917888.pdf