Wasko, Adriane Pinto [UNESP]Severino, Fábio E. [UNESP]Presti, Flávia T. [UNESP]Poletto, Andréia B. [UNESP]Martins, Cesar [UNESP]2014-05-202014-05-202007-01-01Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 4, p. 1089-1092, 2007.1415-4757http://hdl.handle.net/11449/30502An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.1089-1092engactin geneamino acid residuesgene structureNile tilapianucleotide sequenceOreochromis niloticusArtigo10.1590/S1415-47572007000600010S1415-47572007000600010Acesso aberto2-s2.0-37649020453S1415-47572007000600010.pdf192686391737890988588006994253520000-0003-4600-03670000-0003-3534-974X