Barbosa, Adelton AndradeDel Carlo, Ricardo JunqueiraGalvão, Simone RezendeLouzada, Mário Jefferson Quirino [UNESP]Franco, Frederico Souzalima CaldoncelliFernandes, Bárbara BragaCarneiro-Junior, Miguel AraújoRamos, Regiane Maria SoaresDrummond, Lucas RiosNatali, Antonio Jose2022-04-292022-04-292012-07-01Bioscience Journal, v. 28, n. 4, p. 660-666, 2012.1516-37251981-3163http://hdl.handle.net/11449/232104We investigated the importance of daily free activity in the cage and body weight gain during the recovering of bone structural and mechanical properties in growing rats after hindlimb unloading. Eight-week-old male Wistar rats were randomly divided into control (CG, n=24) and suspended (SG, n=24) groups. Animals from SG underwent a four-week hindlimb unloading period by tail-suspension. Animals from CG and those from SG after release were kept in collective cages and sacrificed at the age of 12, 16 and 20 weeks. Both femurs were removed and its area, bone mineral density (BMD), resistance to failure and stiffness were determined. Four-week hindlimb unloading decreased (p<0.05) body weight (CG, 373.00 ± 9.47 vs. SG, 295.86 ± 9.19 g), BMD (CG, 0.19 ± 0.01 vs. SG, 0.15 ± 0.01 g/cm 2), bone resistance to failure (CG, 147.75 ± 5.05 vs. SG, 96.40 ± 5.95 N) and stiffness (CG, 0.38 ± 0.01 vs. SG, 0.23 ± 0.02 N/m). Eight weeks of free activity in cage recovered (p>0.05) the body weight (CG, 472.75 ± 14.11 vs. SG, 444.75 ± 18.91 g), BMD (CG, 0.24 ± 0.01 vs. SG, 0.22 ± 0.01 g/cm 2), bone resistance to failure (CG, 195.73 ± 10.06 vs. SG, 178.45 ± 8.48 N) and stiffness (CG, 0.56 ± 0.02 vs. SG, 0.47± 0.03 N/m) of SG animals. Body weight correlated strongly with bone structural and mechanical properties (p<0.0001). In conclusion, free activity in the cage associated with body weight gain restored bone structural and mechanical properties in growing rats after hindlimb unloading.660-666engBone mineral densityBone stiffnessOsteopeniaArtigo2-s2.0-84867797626