Ravalli, A.Pilon Dos Santos, G. [UNESP]Ferroni, M.Faglia, G.Yamanaka, Hideko [UNESP]Marrazza, G.2014-05-272014-05-272013-01-01Sensors and Actuators, B: Chemical, v. 179, p. 194-200.0925-4005http://hdl.handle.net/11449/74197This paper describes the optimisation and the analytical performances of a label-free impedimetric immunosensor for the detection of tumour marker CA125 based on gold nanoparticles modified screen-printed graphite electrode. Experimental conditions of each step for the developed immunosensor were studied and optimised. The immunosensor response varied linearly (r2 = 0.996) with antigen concentration between 0 and 100 U/mL. The estimated detection limit was 6.7 U/mL. The electrochemical immunosensor allowed unambiguous identification of CA125, while no significant non-specific signal was detected in the case of all negative controls. The analytical usefulness of the impedimetric immunosensor was finally demonstrated analysing serum samples. © 2012 Elsevier B.V. All rights reserved.194-200engCA125Gold nanoparticlesImpedimetric immunosensorScreen-printed electrodeTumour markerAnalytical performanceDetection limitsElectrochemical immunosensorsExperimental conditionsGold NanoparticlesImpedimetric immunosensorsScreen printed electrodesGoldGraphite electrodesMetal nanoparticlesTumorsImmunosensorsArtigo10.1016/j.snb.2012.10.017WOS:000316588100024Acesso restrito2-s2.0-848809213001923726000036625