Bussmann, André RobertoMarton Filho, Marcos AntônioMódolo, Marília PinheiroMódolo, Renata PinheiroAmado, PatríciaDomingues, Maria Aparecida Custódio [UNESP]Castiglia, Yara Marcondes Machado [UNESP]Módolo, Norma Sueli Pinheiro [UNESP]2015-02-022015-02-022014-08-01Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 8, p. 515-521, 2014.0102-8650http://hdl.handle.net/11449/114483PURPOSE: To investigate whether allopurinol exerts a protective effect on kidneys by measuring new kidney injury biomarkers (NGALp, NGALu, KIM 1 and IL 18) and analysing the renal function and histology in uninephrectomised rats subjected to ischaemia-reperfusion injury. METHODS: Thirty two Wistar rats were randomly allocated to four groups: Sham (S): laparotomy; Control (C): laparotomy and ischaemia-reperfusion in the left kidney; Control Allopurinol (CA): laparotomy and allopurinol at a dose of 100mg·kg 1·d 1; and Allopurinol (A): laparotomy ischaemia-reperfusion in the left kidney and allopurinol at a dose of 100mg·kg 1·d 1. The NGALp, NGALu, KIM 1, IL 18 and creatinine levels and the kidney histology were analysed. The significance level was established as p<0.05. RESULTS: Creatinine level increased in all the groups, with A ≈ C > S ≈ CA. The NGALp, NGALu and IL 18 levels exhibited similar behaviour in all the groups. KIM 1 was higher in group A than C and showed intermediate values in groups S and CA. Severity of injury in the left kidney was greater in groups C and A compared to S and CA. CONCLUSION: Allopurinol did not exert protective or damaging effects on the kidneys of rats subjected to ischaemia-reperfusion injury.515-521engIschemiaAcute Kidney InjuryAllopurinolInterleukin-18Biomarkers, PharmacologicalRatsArtigo10.1590/S0102-86502014000800006S0102-86502014000800515Acesso abertoS0102-86502014000800515.pdf82235464757240580585723113037140