Ribeiro, Daniel Araki [UNESP]Alves de Lima, Patrícia LepageMarques, Mariângela Esther Alencar [UNESP]Salvadori, Daisy Maria Favero [UNESP]2014-05-272014-05-272006-12-01Brazilian Dental Journal, v. 17, n. 2, p. 91-94, 2006.0103-64401806-4760http://hdl.handle.net/11449/69288Fluoride has widely been used in Dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury on genetic apparatus. Genotoxicity tests constitute an important part of cancer research for risk assessment of potential carcinogens. In this study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Mouse lymphoma and human fibroblast cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 μg/mL for 3 h at 37μC. The results pointed out that NaF in all tested concentrations did not contribute to DNA damage as depicted by the mean tail moment and tail intensity for both cellular types assessed. These findings are clinically important because they represent a valuable contribution for evaluation of the potential health risk associated with exposure to agents usually used in dental practice.91-94engComet assayHuman fibroblastsMouse lymphoma cellsSodium fluorideanticaries agentfluoride sodiumanalysis of varianceanimalcomet assayDNA damagedrug effectfibroblastgeneticshumanlymphomamousepathologyAnalysis of VarianceAnimalsCariostatic AgentsComet AssayDNA DamageFibroblastsHumansLymphomaMiceSodium FluorideArtigo10.1590/S0103-64402006000200001S0103-64402006000200001Acesso aberto2-s2.0-346488189652-s2.0-34648818965.pdf50511187529809037528116925519142