Cardoso, Tereza C. [UNESP]Ferrari, Heitor F. [UNESP]Bregano, Livia C. [UNESP]Silva-Frade, Camila [UNESP]Rosa, Ana Carolina G. [UNESP]Andrade, Alexandre Lima de [UNESP]2013-09-302014-05-202013-09-302014-05-202010-12-01Molecular and Cellular Probes. London: Academic Press Ltd- Elsevier B.V. Ltd, v. 24, n. 6, p. 415-417, 2010.0890-8508http://hdl.handle.net/11449/14840A sensitive reverse-transcription loop-mediated isothermal amplification (RI-LAMP) assay was developed for the rapid visual detection of turkey coronavirus (TCoV) infection The reaction is performed in one step in a single tube at 65 degrees C for 45 min with hydroxynaphthol blue (HNB) dye added prior to amplification The detection limit of the RI-LAMP assay was approximately 10(2) EID50/50 mu l TCoV genome and no cross-reaction with other avian viruses was observed The assay was evaluated further in tissue suspensions prepared from the ileum and ileum-caecal junctions of infected turkey embryos 100% of these samples were positive in the RI-LAMP assay All individual feces samples collected in the field were considered positive by both conventional RT-PCR and RI-LAMP In conclusion RI-LAMP with HNB dye was shown to be a sensitive simple assay for the rapid diagnosis of TCoV infection either directly from feces or in association with virus isolation methods (C) 2010 Elsevier Ltd All rights reserved415-417engTurkey coronavirusHydroxynaphthol blueLAMP assaySpike geneArtigo10.1016/j.mcp.2010.08.003WOS:000283970200015Acesso restrito6404176495306171