Marques, Mariângela Esther Alencar [UNESP]Coelho, Kunie Iabuki Rabello [UNESP]Sotto, M. N. [UNESP]Bacchi, C. E. [UNESP]2014-05-272014-05-271992-01-01Journal of Clinical Pathology, v. 45, n. 12, p. 1089-1093, 1992.0021-9746http://hdl.handle.net/11449/64210Aims: To compare the efficacy of histochemical and immunohistochemical methods in detecting forms of Sporothrix schenckii in tissue. Methods: Thirty five cutaneous biopsy specimens from 27 patients with sporotrichosis were stained by histochemical haemotoxylin and eosin, periodic acid Schiff, and Gomori's methenamine silver methods and an immunohistochemical (avidin-biotin complex immunoperoxidase) (ABC) technique associated with a newly produced rabbit polyclonal antibody anti-Sporothrix schenckii. Results: A total of 29 (83%) cases were positive by the ABC method used in association with anti-Sporothrix schenckii rabbit polyclonal antibodies. Histochemical methods, using silver staining, periodic acid Schiff, and conventional haematoxylin and eosin detected 37%, 23%, and 23% of forms of S schenckii, respectively. The ABC technique was significantly more reliable than periodic acid Schiff and silver staining techniques. Conclusions: It is concluded that immunostaining is an easy and rapid method which can efficiently increase the accuracy of the diagnosis of sporotrichosis in human tissue.1089-1093enghistochemistryhuman tissueimmunohistochemistryskin biopsysporotrichosisAntibodies, FungalHistocytochemistryImmunohistochemistrySensitivity and SpecificitySporothrixSporotrichosisArtigo10.1136/jcp.45.12.1089WOS:A1992KD36800011Acesso aberto2-s2.0-00266900962-s2.0-0026690096.pdf7528116925519142