Zanelli, Cleslei Fernando [UNESP]Maragno, Ana L. C.Gregio, Ana P. B.Komili, SuzannePandolfi, Jose R.Mestriner, Carlos A.Lustri, Wilton R.Valentini, Sandro Roberto [UNESP]2014-05-202014-05-202006-10-06Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 348, n. 4, p. 1358-1366, 2006.0006-291Xhttp://hdl.handle.net/11449/7744The putative translation factor eIF5A is essential for cell viability and is highly conserved from archebacteria to mammals. Although this protein was originally identified as a translation initiation factor, subsequent experiments did not support a role for eIF5A in general translation. In this work, we demonstrate that eIF-5A interacts with structural components of the 80S ribosome, as well as with the translation elongation factor 2 (eEF2). Moreover, eIF5A is further shown to cofractionate with monosomes in a translation-dependent manner. Finally, eIF5A mutants show altered polysome profiles and are sensitive to translation inhibitors. Our results re-establish a function for eIF5A in translation and suggest a role for this factor in translation elongation instead of translation initiation. (c) 2006 Elsevier B.V. All rights reserved.1358-1366engeIF5AribosometranslationeEF2P0L11hypusineprotein synthesisArtigo10.1016/j.bbrc.2006.07.195WOS:000240425600020Acesso restrito533325035504981415256654089001950000-0001-7831-1149