Crepaldi-Alves, S. C. [UNESP]Carneiro, E. M. [UNESP]Bosqueiro, José Roberto [UNESP]Boschero, A. C.2014-05-272014-05-271997-03-01Brazilian Journal of Medical and Biological Research, v. 30, n. 3, p. 359-361, 1997.0100-879Xhttp://hdl.handle.net/11449/65057We studied the synergistic effect of glucose and prolactin (PRL) on insulin secretion and GLUT2 expression in cultured neonatal rat islets. After 7 days in culture, basal insulin secretion (2.8 mM glucose) was similar in control and PRL-treated islets (1.84 ± 0.06% and 2.08 ± 0.07% of the islet insulin content, respectively). At 5.6 and 22 mM glucose, insulin secretion was significantly higher in PRL-treated than in control islets, achieving 1.38 ± 0.15% and 3.09 ± 0.21 % of the islet insulin content in control and 2.43 ± 0.16% and 4.31 ± 0.24% of the islet insulin content in PRL-treated islets, respectively. The expression of the glucose transporter GLUT2 in B-cell membranes was dose-dependently increased by exposure of the islet to increasing glucose concentrations. This effect was potentiated in islets cultured for 7 days in the presence of 2 μg/ml PRL. At 5.6 and 10 mM glucose, the increase in GLUT2 expression in PRL-treated islets was 75% and 150% higher than that registered in the respective control. The data presented here indicate that insulin secretion, induced by different concentrations of glucose, correlates well with the expression of the B-cell-specific glucose transporter GLUT2 in pancreatic islets.359-361engGlucoseGlucose transporterPancreatic isletsProlactinglucoseglucose transporterglucose transporter 2prolactinanimalbiosynthesisculture techniquemetabolismpancreas isletphysiologyratAnimalsCell Culture TechniquesGlucose Transporter Type 2Islets of LangerhansMonosaccharide Transport ProteinsRatsArtigo10.1590/S0100-879X1997000300008S0100-879X1997000300008WOS:A1997WQ16600008Acesso aberto2-s2.0-00310891242-s2.0-0031089124.pdf