de Oliveira, Analú Barros [UNESP]Ferrisse, Túlio Morandin [UNESP]França, Gabriela Gomes [UNESP]de Annunzio, Sarah Raquel [UNESP]Kopp, Willian [UNESP]Fontana, Carla Raquel [UNESP]Brighenti, Fernanda Lourenção [UNESP]2023-07-292023-07-292023-02-01Pathogens, v. 12, n. 2, 2023.2076-0817http://hdl.handle.net/11449/248414The synergic effect of Streptococcus mutans and Candida albicans increases dental caries severity. Antimicrobial photodynamic therapy (aPDT) is a non-invasive treatment for antimicrobial aspects. However, the current photosensitizers (PS) have many downsides for dental applications. This study aimed to evaluate the efficiency of two different Brazilian green propolis (BGP-AF and BGP-AG) as PS for aPDT against these microorganisms. A single-species biofilm was irradiated with crude extracts and their fractions and controls. Such extracts showed the best results and were evaluated in dual-species biofilms. Photodegradation, reactive oxygen species (ROS), cytotoxicity, and color stability assays were also investigated. Reductions higher than 3 log10 CFU/mL (p < 0.0001) occurred for crude BGP in single- and dual-species biofilms. Singlet oxygen was produced in BGP (p < 0.0001). BGP-mediated aPDT delayed S. mutans and C. albicans regrowth after 24 h of treatment (p < 0.0001). Both BGP did not change the color of dental materials (p > 0.05). BGP-AF-mediated aPDT showed 72.41% of oral keratinocyte viability (p < 0.0001). BGP extracts may be used in aPDT against S. mutans and C. albicans. Specifically, BGP-AF may represent a promising PS for dental applications.engantimicrobial photodynamic therapyBrazilian green propoliscariogenic microorganismsnatural photosensitizerArtigo10.3390/pathogens120201552-s2.0-85148759659