Fernandes, Flávio C. Bedatty [UNESP]Andrade, Juliana R. [UNESP]Bueno, Paulo R. [UNESP]2019-10-062019-10-062019-07-15Sensors and Actuators, B: Chemical, v. 291, p. 493-501.0925-4005http://hdl.handle.net/11449/190112An electro-active nanoscale composite was engineered to serve as an alternative to monolayers in a capacitive biosensing platform. An electrochemical impedance-derived spectroscopic method was applied to analyse the response of this nanoscale (c.a. 9 nm thickness) composite to detect C-reactive protein. The composite was successfully assembled over a conductive electrode and built up by conjugating graphene oxide sheets into a self-assembled 11-ferrocenyl-undecanethiol structure, forming a molecular composite concomitantly coated with zwitterionic methacrylate-based monomers that served as the non-fouling component of the composite. Finally, the functionalization of a composite with receptors allowed the successful testing of its electro-analytical ability to detect C-reactive protein in both phosphate-buffered saline and neat human serum samples.493-501engElectrochemical impedance spectroscopyImmittance function electro-analysisLow-foulingNanoscale compositeZwitterionic compoundArtigo10.1016/j.snb.2019.01.164Acesso aberto2-s2.0-85061456424