André, Marcos Rogério [UNESP]Dumler, John StephenHerrera, Heitor M.Gonçalves, Luiz R. [UNESP]Sousa, Keyla C. M. de [UNESP]Scorpio, Diana GerardiSantis, Ana Cláudia Gabriela Alexandre de [UNESP]Domingos, Iara HelenaMacedo, Gabriel Carvalho deMachado, Rosangela Zacarias [UNESP]2015-12-072015-12-072015Journal Of Feline Medicine And Surgery, p. 1-9, 2015.1532-2750http://hdl.handle.net/11449/131427The objective of this study was to develop a quantitative 5' nuclease real-time polymerase chain reaction (PCR) assay to diagnose infections caused by Bartonella species. Between January and April 2013 whole blood samples were collected by convenience from 151 cats (86 domiciled and 65 stray cats). The feline blood samples were subjected to a novel quantitative 5' nuclease real-time PCR (qPCR) for Bartonella species targeting the nictonamide adenine dinucleotide dehydrogenase gamma subunit (nuoG) and conventional PCR assays targeting intergenic transcribed spacer, ribC, gltA, pap31 and rpoB, followed by sequencing and basic local alignment search tool analysis. The qPCR assay detected as few as 10 copies of plasmid per reaction. Forty-six (54.4% domiciled and 45.6% stray cats) of 151 sampled cats showed positive results in nuoG qPCR for Bartonella species. The absolute quantification of nuoG Bartonella DNA in sampled cats ranged from 1.1 × 10(4) to 1.3 × 10(4). Eighteen (39.1%) of 46 positive samples in the qPCR were also positive in conventional PCR assays. The sequencing confirmed that Bartonella henselae and Bartonella clarridgeiae circulate in cats in midwestern Brazil. The present work provides details of a novel qPCR assay to diagnose infections caused by Bartonella species.1-9engArtigo10.1177/1098612X15593787Acesso restrito3254990612451836913989989558051326138812