Hutson, R. A.Thompson, D. E.Lawson, P. A.Schockenitturino, R. P.Bottger, E. C.Collins, M. D.2014-05-202014-05-201993-01-01Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. Dordrecht: Springer, v. 64, n. 3-4, p. 273-283, 1993.0003-6072http://hdl.handle.net/11449/33457The phylogenetic interrelationships of members of the Clostridium botulinum complex of species was investigated by direct sequencing of their 16S rRNA genes. Comparative analysis of the 16S rRNA sequences demonstrated the presence of four phylogenetically distinct lineages corresponding to: i) proteolytic C. botulinum types Al B, and F, and C. sporogenes, ii) saccharolytic types B, E and F, iii) types C and D and C. novyi type A, and iv) type G and C. subterminale. The phylogenetic groupings obtained from the 16S rRNA were in complete agreement with the four divisions recognised within the 'species complex' on the basis of phenotypic criteria.273-283eng16S RIBOSOMAL-RNACLOSTRIDIUM BOTULINUMPHYLOGENYTAXONOMYArtigo10.1007/BF00873087WOS:A1993NN68400006Acesso restrito