Garcia, M.Sertorio, S. P.Alves, G. J.Chate, S. C.Carneiro, R.Lallo, M. A.2014-05-202014-05-202004-07-01Pesquisa Veterinaria Brasileira. Rio Janeiro: Revista Pesquisa Veterinaria Brasileira, v. 24, n. 3, p. 115-119, 2004.0100-736Xhttp://hdl.handle.net/11449/31425Cyclophosphamide (CY) was used to evaluate the effect on the immune system of sheep. Castred adult rams were divided into 3 groups, with 6 animals each one. Group I (day 0) and Group II (day 1) were treated with CY (40 mg/kg, single dose, IV), and Group III was not treated and remained as control. All groups were immunized on day 0 with B19 brucellosis vaccine. on day 6, all animals were bled and serum agglutination test for brucellosis antibodies detection was performed. During 7 days blood lymphocyte counts and electrophoresis gammaglobulin dosage were daily performed. The results showed statistical decrease of immune response. Low serum titers of brucellosis antibodies were found in Groups I and II, and lymphopenia and hypogammaglobulinemia were also found in these groups. A high mortality rate (40%) occurred in the treated animals.115-119porimmunosupressioncyclophosfamideimmunologysheepArtigo10.1590/S0100-736X2004000300001S0100-736X2004000300001WOS:000225468400001Acesso abertoWOS000225468400001.pdf